Diagnostic kits
database
Tomato brown rugose fruit virus (ToBRFV)
Real-Time PCR
Organism/pest:
Tomato brown rugose fruit virus (ToBRFV)
Taxonomic group:
Virus
Manufacturer:
Bioreba
Type of detection:
Molecular
Type of test:
Molecular test
Type of method:
Real-Time PCR
Description of the test:
The qPCR ToBRFV set enables the detection of Tomato brown rugose fruit virus (ToBRFV) with a triplex real-time RT-PCR. Viral RNA of ToBRFV, extracted from tomato or pepper samples, is amplified with two distinct primer pairs in a one-step RT-PCR reaction.
Type of sample:
Leaves; seeds; fruits
Number of target:
1
Target:
Tomato brown rugose fruit virus (ToBRFV)
Available extraction method:
For the extraction of RNA it is recommended to use standard RNA extraction protocols established for plant material. Upon request, BIOREBA can recommend an optimal extraction method.
Available extraction method PDF:
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Scope:
The qPCR ToBRFV set is validated for the simultaneous detection (multiplex) of tomato brown rugose fruit virus (ToBRFV) and a cytochrome oxidase (COX) gene sequence which serves as internal positive control (IPC) by one-step real- time RTPCR. Suitable tissues are tomato/pepper leaves, seeds and fruit samples. Samples of up to 1000 seeds can be pooled for RNA extraction and analyzed according to this qPCR protocol.
EPDIA validation datasheet:
Other validation data:
- Validation report Bioreba
Other Validation data PDF:
References:
(1) Salem N., Mansour A., Ciuffo M., Falk B., Turina M. 2016. A new tobamovirus infecting tomato crops in Jordan. Arch Virol. 161(2):503–6.
(2) Luria, N., Smith, E., Reingold, V., Bekelman, I., Lapidot, M., Levin, I., Elad, N., Tam, Y., Sela, N., Abu-Ras, A. and Ezra, N., 2017. A new Israeli Tobamovirus isolate infects tomato plants harboring Tm-22 resistance genes. PloS one, 12(1), p.e0170429.
(3) Menzel W., Knierim D., Winter S., Hamacher J., Heupel M. 2019. First report of tomato brown rugose fruit virus infecting tomato in Germany. New Disease Reports 39:1.
(2) Luria, N., Smith, E., Reingold, V., Bekelman, I., Lapidot, M., Levin, I., Elad, N., Tam, Y., Sela, N., Abu-Ras, A. and Ezra, N., 2017. A new Israeli Tobamovirus isolate infects tomato plants harboring Tm-22 resistance genes. PloS one, 12(1), p.e0170429.
(3) Menzel W., Knierim D., Winter S., Hamacher J., Heupel M. 2019. First report of tomato brown rugose fruit virus infecting tomato in Germany. New Disease Reports 39:1.
References PDF:
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User guidelines/protocol/manual:
MSDS sheet:
Others:
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Video:
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Website:
Contact:
Country of production:
Web shop:
www.bioreba.com
Product code:
889600 (qPCR ToBRFV set 96); 889200 (qPCR ToBRFV set 192)
Available kit format:
96 or 192 reactions
Components:
889600 (qPCR ToBRFV set 96) and 889200 (qPCR ToBRFV set 192)
Include:
Taq Master Mix (2x) Art. No 831412
RT Master Mix (50x) Art. No. 830414
Primers/Probes Mix_ToBRFV/COX (10x) Art. No 880100
Nuclease-free water Art. No. T143.4
Include:
Taq Master Mix (2x) Art. No 831412
RT Master Mix (50x) Art. No. 830414
Primers/Probes Mix_ToBRFV/COX (10x) Art. No 880100
Nuclease-free water Art. No. T143.4
Other information:
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