Type of detection:
Type of method:
Description of the test:
The prunus necrotic ringspot virus (PNRSV) DAS-ELISA Test was developed to detect isolates from plum, apricot or peach. PNRSV (4) has a fairly wide host range among dicotyledonous plants. There is no known insect vector, the virus is transmitted by pollen to seed and to pollinated plants. PNRSV causes necrotic ringspot in many species of Prunus, often with subsequent recovery from symptoms. It causes sweet cherry rugose mosaic, almond calico, a mosaic disease of rose, and is common in hop, in which it is symptomless or associated with ring and band mosaic.
Type of sample:
leaves; flowers; dormant buds; bark of young shoots
Prunus necrotic ringspot virus (PNRSV)
Available extraction method:
Samples are homogenised 1:20 (w/v) in extraction buffer «General». The HOMEX homogenizer in combination with BIOREBA extraction bags is recommended for sample preparation.
Available extraction method PDF:
These DAS-ELISA reagents (1) were made against an isolate of PNRSV (isolate 1/12) from plum (2). The reagents do not distinguish between isolates from plum, cherry, apricot or peach. The virus can generally be detected in fruit trees without difficulties throughout the whole year, either in leaves, flowers, dormant buds or bark of young shoots (3). Samples are homogenised 1:20 (w/v) in extraction buffer «General» (Art. No. 110120). The product was developed in cooperation with the Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.