Diagnostic kits
database

Potato virus A (PVA); potato virus M (PVM)

Real-Time PCR

Organism/pest:
Potato virus A (PVA); potato virus M (PVM)
Taxonomic group:

Virus

Manufacturer:
Bioreba
Bioreba
Potato virus A (PVA); potato virus M (PVM)
Type of detection:
Molecular
Type of test:
Molecular test
Type of method:
Real-Time PCR
Description of the test:
The qPCR set enables the simultaneous detection (multiplex) of potato virus A (PVA) and potato virus M (PVM) with real-time PCR.
Type of sample:
leaves; tubers (peel, stolon, sprouts);
Number of target:
2
Target:
Potato virus A (PVA); potato virus M (PVM)
Available extraction method:
It is highly recommended to use the BIOREBA “Potato DNA/RNA rapid extraction” (included in the qPCR kits) for the extraction of RNA. The extraction method with “Potato DNA/RNA rapid extraction” has been validated and accredited according to ISO/IEC 17025. The method has been approved for the certification of seed potato.
Available extraction method PDF:
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Scope:
The qPCR set is validated for the simultaneous detection (multiplex) of potato virus A (PVA), potato virus M (PVM) and internal control (IC) in real- time PCR. Suitable tissues are potato leaves and tuber samples (peel, stolon and sprouts). Samples of up to 25 dormant tubers can be pooled for RNA extraction and analyzed according to this qPCR protocol.
EPDIA validation datasheet:
-
Other validation data:
- Proficiency Tests organized by "Bayerische Landesanstalt für Landwirtschaft" in 2018, 2020 and 2021
References:
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References PDF:
-
Contact:
Country of production:

Switzerland

Web shop:
www.bioreba.ch
Product code:
849200 (qPCR PVA/PVM set 192); 849210 (qPCR PVA/PVM set 192/10); 849225 (qPCR PVA/PVM kit 192/25); 849600 (qPCR PVA/PVM set 96); 849610 (qPCR PVA/PVM set 96/10); 849625 (qPCR PVA/PVM set 96/25)
Available kit format:
96 reactions (pools up to 1, 10, or 25 samples); 192 reactions (pool up to 1, 10, or 25 samples)
Components:
Set format contains:
- Taq Master Mix (2x)
- RT Master Mix (50x)
- Primers/Probes/IC Mix_PVA/PVM/IC (10x)
- Nuclease-free water

Kit format contains:
- Set 96 or 192
- Potato DNA/RNA rapid extraction set
Other information:
-

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