Type of detection:
Type of test:
Type of method:
Description of the test:
The qPCR set enables the simultaneous detection (multiplex) of potato virus A (PVA) and potato virus M (PVM) with real-time PCR.
Type of sample:
leaves; tubers (peel, stolon, sprouts);
Potato virus A (PVA); potato virus M (PVM)
Available extraction method:
It is highly recommended to use the BIOREBA “Potato DNA/RNA rapid extraction” (included in the qPCR kits) for the extraction of RNA. The extraction method with “Potato DNA/RNA rapid extraction” has been validated and accredited according to ISO/IEC 17025. The method has been approved for the certification of seed potato.
Available extraction method PDF:
The qPCR set is validated for the simultaneous detection (multiplex) of potato virus A (PVA), potato virus M (PVM) and internal control (IC) in real- time PCR. Suitable tissues are potato leaves and tuber samples (peel, stolon and sprouts). Samples of up to 25 dormant tubers can be pooled for RNA extraction and analyzed according to this qPCR protocol.