Diagnostic kits
database

Molecular

Molecular test

Real-Time PCR

The qPCR set enables the simultaneous detection (multiplex) of potato virus A (PVA) and potato virus M (PVM) with real-time PCR.

leaves; tubers (peel, stolon, sprouts);

2

Potato virus A (PVA); potato virus M (PVM)

It is highly recommended to use the BIOREBA “Potato DNA/RNA rapid extraction” (included in the qPCR kits) for the extraction of RNA. The extraction method with “Potato DNA/RNA rapid extraction” has been validated and accredited according to ISO/IEC 17025. The method has been approved for the certification of seed potato.

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The qPCR set is validated for the simultaneous detection (multiplex) of potato virus A (PVA), potato virus M (PVM) and internal control (IC) in real- time PCR. Suitable tissues are potato leaves and tuber samples (peel, stolon and sprouts). Samples of up to 25 dormant tubers can be pooled for RNA extraction and analyzed according to this qPCR protocol.

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- Proficiency Tests organized by "Bayerische Landesanstalt für Landwirtschaft" in 2018, 2020 and 2021

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www.bioreba.ch

849200 (qPCR PVA/PVM set 192); 849210 (qPCR PVA/PVM set 192/10); 849225 (qPCR PVA/PVM kit 192/25); 849600 (qPCR PVA/PVM set 96); 849610 (qPCR PVA/PVM set 96/10); 849625 (qPCR PVA/PVM set 96/25)

96 reactions (pools up to 1, 10, or 25 samples); 192 reactions (pool up to 1, 10, or 25 samples)

Set format contains:
- Taq Master Mix (2x)
- RT Master Mix (50x)
- Primers/Probes/IC Mix_PVA/PVM/IC (10x)
- Nuclease-free water

Kit format contains:
- Set 96 or 192
- Potato DNA/RNA rapid extraction set

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