Type of detection:
Type of method:
Lateral Flow Immunochromatography
Description of the test:
PPV AgriStrip is a rapid assay for the detection of Plum pox virus (PPV). The PPV AgriStrip is a lateral-flow immunochromatographic test based on an antigen-antibody reaction that is initiated by inserting the strip into the sample extract. The sample liquid migrates upwards and red colored lines will become visible within a few minutes. Both test and control lines become visible with positive extracts (containing PPV), whereas negative samples produce only the upper control line. Intense coloration is reached within 10 – 15 min and the result can be registered. Dried test strips can be kept as permanent records.
Type of sample:
Blossom; symptomatic leaves; petioles
Plum pox virus (PPV)
Available extraction method:
Place a piece of 2.5 x 3 cm of leaf (that is approx. 0.15 g) or three blossoms in an extraction bag, then add 3 ml of extraction buffer B with a disposable pipette. Grind the tissue with a handheld homogenizer on a flat surface with few movements for not more than 3-4 seconds to obtain an 1:20 (w/v) extract.
Available extraction method PDF:
The rapid assay PPV AgriStrip is produced by BIOREBA for identification of PPV (Sharka) in Prunus sp. showing symptoms such as chlorotic spots, blotches, bands, rings or line patterns on leaves. Later symptoms include uneven ripening, blotching and rings on fruits. The concentration of PPV in tissues of fruit trees may vary considerably. For example, in peach and abricot trees, the concentration varies even within the same leaf.