Type of detection:
Serological
Type of method:
DAS-ELISA
Description of the test:
DAS-ELISA using polyclonal antiserum from rabbit, prepared against TMV, ToMV and PMMoV.
Detects many Tobamoviruses of Main reaction with TMV, ToMV, PMMoV, also reacts with TMGMV, ToBRFV, ToMMV, RMV, BePMV, slight reaction with TYCV.
Type of sample:
Leaves and seed of Tomato and Pepper and other significant host plants.
Target:
Tobacco Mosaic Virus, Tomato Mosaic Virus, Pepper Mild Mottle Virus
Available extraction method:
According to standard DAS-ELISA protocol. For seed samples for example see protocols by the International Seed Federation.
Available extraction method PDF:
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Scope:
Simultaneaous screening of plant and seed material for the presence of TMV, ToMV, and PMMoV in one ELISA test. Can also be used to detect several other Tobamoviruses.
Not suitable for discrimination of the viruses.