Diagnostic kits
database
Ralstonia solanacearum
End-point PCR
Organism/pest:
Ralstonia solanacearum
Taxonomic group:
Bacteria
Manufacturer:
Loewe
Type of detection:
Molecular
Type of test:
Molecular test
Type of method:
End-point PCR
Description of the test:
PCR for the detection of Ralstonia solanacearum isolates
Type of sample:
Bacterial DNA from plants or from pre-cultured colonies can be extracted using a standard method or a commercially available DNA extraction kit to receive high-quality DNA free of PCR inhibitors. The extracted DNA can be used directly for testing.
Number of target:
1
Target:
Ralstonia solanacearum
Available extraction method:
Plant DNA Extraction Kit produced for LOEWE® by Analytic Jena, LOEWE® catalogue no. 0845DNA/050
Available extraction method PDF:
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Scope:
Highly sensitive and specific detection of Ralstonia solanacearum
EPDIA validation datasheet:
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Other validation data:
For in-house validation data please see the attached product specifications.
Other Validation data PDF:
References:
(1) K. H. Pastrik & E. Maiss 2000; Detection of Ralstonia solanacearum in potato tubers by Polymerase Chain Reaction; J. Phytopathology 148: 619-626.
(2) Ralstonia solanacearum, EPPO Bulletin 34, Septmeber 2003.
(2) Ralstonia solanacearum, EPPO Bulletin 34, Septmeber 2003.
References PDF:
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User guidelines/protocol/manual:
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MSDS sheet:
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Others:
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Video:
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Website:
Contact:
Country of production:
Web shop:
Product code:
08056S/100
08056C/100
08056C/100
Available kit format:
100 reactions
Components:
Complete kits contain all components to perform PCR reaction, i.e. Premix (pathogen specific primers and dNTPs), PCR Reaction Buffer, DNA-Polymerase (Hot start)*, Positive control, Negative control and PCR-grade water.
PCR Sets are composed of Premix (pathogen specific primers and dNTPs), Positive control and Negative control.
*produced for LOEWE® by Solis BioDyne
PCR Sets are composed of Premix (pathogen specific primers and dNTPs), Positive control and Negative control.
*produced for LOEWE® by Solis BioDyne
Other information:
Kit components are stable during shipping. Upon receipt, store all components below -18°C! After rehydration of the premix and controls, store below -18°C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, controls and primer mix should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the certificate.