Diagnostic kits
database
Tobamovirus Broad Range
End-point PCR
Organism/pest:
Tobamovirus Broad Range
Taxonomic group:
Virus
Manufacturer:
Loewe
Type of detection:
Molecular
Type of test:
Molecular test
Type of method:
End-point PCR
Description of the test:
One-Tube One-Step RNA PCR for the generic detection of tobamoviruses
Type of sample:
RNA extracted from plant material
Number of target:
0
Target:
Tobamoviruses
Available extraction method:
Plant RNA Extraction Kit produced for LOEWE® by Analytic Jena, LOEWE® catalogue no. 0945RNA/050
Available extraction method PDF:
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Scope:
Generic and sensitive detection of tobamoviruses
EPDIA validation datasheet:
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Other validation data:
For in-house validation data please see the attached product specifications.
Other Validation data PDF:
References:
Dovas CI, Efthimiou K and Katis NI. Generic detection and differentiation of tobamoviruses by a spot nested RT-PCR-RFLP using dI-containing primers along with homologous dG-containing primers (Journal of Virological Methods, 2004)
References PDF:
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User guidelines/protocol/manual:
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MSDS sheet:
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Others:
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Video:
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Website:
Contact:
Country of production:
Web shop:
Product code:
09184C/100
Available kit format:
100 reactions
Components:
Complete kits contain all components to perform PCR reaction, i.e. Premix (pathogen specific primers and dNTPs), RNA-PCR Reaction Buffer (includes MgCl2), DNA-Polymerase (Hot start)*, Reverse-Transcriptase (M-MLV, RNase H-)*, Positive control (DNA amplicon , specific only for primers provided with 09184C/100), Negative control (DNA from healthy plants), 100mM DTT (Dithiothreitol) and PCR-grade water.
*produced for LOEWE® by Solis BioDyne
*produced for LOEWE® by Solis BioDyne
Other information:
Kit components are stable during shipping. Upon receipt, store all components below -18°C! After rehydration of the premix and controls, store below -18°C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, controls and primer mix should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the certificate.