Diagnostic kits
database
Xylella fastidiosa
Real-Time PCR
Organism/pest:
Xylella fastidiosa
Taxonomic group:
Bacteria
Manufacturer:
Loewe
Type of detection:
Molecular
Type of test:
Molecular test
Type of method:
Real-Time PCR
Description of the test:
LOEWE®REALTIME
Dye-based Real-Time PCR for the detection of Xylella fastidiosa
Dye-based Real-Time PCR for the detection of Xylella fastidiosa
Type of sample:
Bacterial DNA from plants or from pre-cultured colonies can be extracted using a standard method or a commercially available DNA extraction kit to receive high-quality DNA free of PCR inhibitors. The extracted DNA can be used directly for testing.
Number of target:
1
Target:
Xylella fastidiosa
Available extraction method:
Plant DNA Extraction Kit produced for LOEWE® by Analytic Jena, LOEWE® catalogue no. 0845DNA/050
Available extraction method PDF:
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Scope:
Highly sensitive and specific detection of Xylella fastidiosa
EPDIA validation datasheet:
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Other validation data:
The kit was validated in parallel to a Ring Test that was part of the POnTE project. For detailed validation data please see the attached product specifications.
Other Validation data PDF:
References:
(1) Harper SJ, Ward LI & Clover GRG (2010) Development of LAMP and real-time PCR methods for the rapid detection of Xylella fastidiosa for quarantine and field applications. Phytopathology 100, 1282-1288.
(2) erratum 2013
(3) EPPO Bulletin (2018) 48 (2), 175-218
(2) erratum 2013
(3) EPPO Bulletin (2018) 48 (2), 175-218
References PDF:
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User guidelines/protocol/manual:
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MSDS sheet:
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Others:
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Video:
-
Website:
Contact:
Country of production:
Product code:
06119C/100
Available kit format:
100 reactions
Components:
Primer Mix (pathogen specific primers), 5x Mastermix (SolisGreen qPCR Mix*), Positive control (Synthesized DNA), Negative control (DNA from healthy plants), PCR-grade water
*produced for LOEWE® by Solis BioDyne
*produced for LOEWE® by Solis BioDyne
Other information:
Kit components are stable during shipping. Upon receipt, store all components below -18°C! After rehydration of the premix and controls, store below -18°C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, controls and primer mix should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the certificate.