Diagnostic kits
database

Grapevine Leafroll-associated Virus 2 (GLRaV-2)

TAS-MIX-ELISA

Organism/pest:
Grapevine Leafroll-associated Virus 2 (GLRaV-2)
Taxonomic group:

Virus

Manufacturer:
Sediag
Sediag
Grapevine Leafroll-associated Virus 2 (GLRaV-2)
Type of detection:
Serological
Type of test:
ELISA test
Type of method:
TAS-MIX-ELISA
Description of the test:
ELISA diagnostic kit for GLRaV-2
Type of sample:
Grapevine samples
Number of target:
1
Target:
GLRaV-2
Available extraction method:
Grind 1g of plant material in 10mL of grapevine extraction buffer
Available extraction method PDF:
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Scope:
Detection of GLRaV-2 in plant material.
EPDIA validation datasheet:
Other validation data:
SEDIAG Certificat of perfomance
Other Validation data PDF:
References:
Seddas S., et al 2003. Use of Biosensor Technology (BIACORE) in the production of Monoclonal Antibodies to grapevine Viruses., ICVG BARI, 2003  
Seddas, A. et al.,2000. A monoclonal antibody reveals that grapevine leafroll associated closteroviruses 1 and 3 are serologically related. Plant pathology, 49, 80-85   Walter B., 1997. Sanitary selection of the grapevine : Protocols for the detection of viruses and virus-like diseases. Les Colloques INRA Editions, 86, 225 p.  
Walter B. et Martelli GP., 1997.Clonal and sanitary selection of the grapevine. In Walter B., ed. Sanitary Selection of the grapevine. Paris, France-INRA, 43-96.  
Habili N. et al., 1996. Virus types associated with grapevine leafroll disease in Australia., Annual Technical Issue of Australian Grapegrower & Winemaker, 25-28.  
Greif. et al., 1995. The relationship of grapevine leafroll-associated closterovirus 2 with a graft incompatibility condition of grapevines., Phytopath. Medit., 34, 167-173.  
Van Regenmortel M.H.V. et Dubs MC., 1993. Serological procedures. In Matthews REF, ed. Diagnosis of Plant Virus Diseases. London, UK, 159-214.  
Zimmermann D. et al., 1990a.Characterization and serological detection of four closterovirus particles associated with leafroll disease on grapevine., J. Phytopath., 130, 205-218.  
Zimmermann D. et al., 1990b.Production and characterization of monoclonal antibodies specific to closterovirus-like particles associated with grapevine leafroll disease., J. Phytopath., 130, 277-288.  
Zimmermann D. et al., 1988. Purification des particules virales associées à l’enroulement de la vigne et mise au point d’un protocole ELISA permettant leur détection., Agronomie, 8 (8), 731-741.  
Van Regenmortel M., 1982. Serology and Immunochemistry of Plant Viruses., Academic Press, 302.
References PDF:
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Website:
Contact:
Country of production:

France

Product code:
LR2-LNC
LR2-LPC
LR2-SRA 0500
LR2-SRA 1000
LR2-SRA 5000
LR2-XRA 0500
LR2-XRA 1000
LR2-TRA 0500
Available kit format:
500, 1000 or 5000 tests
Components:
Reagent Set (SRA) : Antibodies (Coating and Conjugate)
Full Kit (XRA) : Antibodies (Coating and Conjugate), Positive control, Negative control, Buffers (Coating, Conjugate, Substrat, Wash and Extraction), Substrate Tablets, ELISA plates.
Kit 480 (TRA) : Antibodies (Coating and Conjugate), Positive control, Negative control, Buffers 1X (Coating, Conjugate, Substrat), Substrate Tablets.
Other information:
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