Type of detection:
Type of test:
Type of method:
Description of the test:
The qPCR set enables the simultaneous detection (multiplex) of potato virus Y (PVY; all strains) and potato leafroll virus (PLRV) with real-time PCR. Viral RNA, extracted from potato samples, is amplified in a one-step RT-PCR reaction. The amplification of the cDNA can be monitored in real time, because the specific probes are labeled with fluorophores (PVY: FAM and PLRV: Cy5). An internal control (IC: JOE) is included for the convenience of the operator. The control shows whether the reverse transcription and the amplification of the RNA worked as intended.
Suitable tissue sources of viral RNA are potato leaves and tubers (peel, stolon, sprouts). It is highly recommended to use the BIOREBA â€œPotato DNA/RNA Rapid Extraction (included in the qPCR kits) for the extraction of RNA. The extraction method with Potato DNA/RNA rapid extraction has been validated and accredited according to ISO/IEC 17025. The method has been approved for the certification of seed potato. The qPCR for the detection of PLRV, PVY and IC was developed and validated in two certification seasons (2014/15) in collaboration with Agroscope, the Swiss centre of excellence for research in the agriculture and food sectors.
Type of sample:
Leaves; peel; stolon; sprouts;
Potato leafroll virus (PLRV); potato virus Y (PVY)
Available extraction method:
It is highly recommended to use the BIOREBA “Potato DNA/RNA Rapid Extraction” (included in the qPCR kits) for the extraction of RNA. The extraction method with “Potato DNA/RNA rapid extraction” has been validated and accredited according to ISO/IEC 17025. The method has been approved for the certification of seed potato.
Available extraction method PDF:
The qPCR set is validated for the simultaneous detection (multiplex) of potato virus Y (PVY), potato leaf roll virus (PLRV) and internal control (IC) in real- time PCR. Suitable tissues are potato leaves and tuber samples (peel, stolon and sprouts). Samples of up to 25 dormant tubers can be pooled for RNA extraction and analyzed according to this qPCR protocol.