Diagnostic kits
database

Serological

ELISA test

DAS-ELISA

DAS-ELISA test to detect PepMV (6). PepMV, a potexvirus, was first reported in pepino (Solanum muricatum) in Peru (2). In Europe, first outbreaks of PepMV in greenhouse tomato have been reported 1999/2000 in the Netherlands, Germany, France, the UK and Spain, subsequently in many European countries as well as in Canada, USA and China. Other solanaceous plants such as potatoes could be infected by artificial inoculation, but so far no PepMV problem has been observed in that crop. Different PepMV genotypes have been recognized (3, 5). Transmission occurs by contact, e.g. by clothes, contaminated tools, fruits and other plant parts, and at very low rate, by seed. PepMV is on the EPPO A2 List and is a regulated pathogen on tomato seeds in the EU. Containment of disease is achieved by sanitary measures, including seed disinfection (e.g. commercial bleach solution, 4) and seed testing. DAS-ELISA (1) is one of the established methods for reliable PepMV detection in plants and seeds.

Leaves; Seeds

1

Pepino mosaic virus (PepMV)

For testing tomato, leaf samples are homogenized 1:20 (w/v) in extraction buffer «General» (Art. No. 110120). For seed testing, the following procedure is recommended: Slightly crush the seeds, e.g. with a hammer, 1:25 (w/v) in extraction buffer «General» (Art. No. 110120), then pre-soak them at least for 4 h (and up to overnight) at 4°C before homogenization (with HOMEX) and testing.

-

The broad-spectrum reagents were made against a selection of PepMV isolates representing all major genotypes and geographic origins; i.e. Ch1 (US1 genotype), Ch2 (CH2 genotype) isolates (3), AZ-2 and TX-1 isolates (EU genotype) (5). The reagents specifically react with PepMV in DAS-ELISA; isolates of all strain groups of different geographic origin are recognized. The virus is detected in leaves and seeds. For testing tomato, leaf samples are homogenized 1:20 (w/v) in extraction buffer «General» (Art. No. 110120). For seed testing, the following procedure is recommended: Slightly crush the seeds, e.g. with a hammer, 1:25 (w/v) in extraction buffer «General», then pre-soak them at least for 4 h (and up to overnight) at 4°C before homogenization (with HOMEX) and testing.

https://www.eppo.int/RESOURCES/eppo_publications/eppo_bulletin

(1) Clark, M.F., and Adams, A.N. 1977. Characteristics of method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. gen. Virol. 34:475-483.
(2) Jones, R.A.C., Koenig, R, and Lesemann, D.-E. 1980. Pepino mosaic virus, a new potexvirus from pepino (Solanum muricatum). Ann. appl. Biol. 94: 61-68.
(3) Ling, K. -S. 2007. Molecular characterization of two Pepino mosaic virus variants from imported tomato seed reveals high levels of sequence identity between Chilean and US isolates. Virus Genes 34:1-8.
(4) Ling, K.-S. 2010. Effectivness of Chemo- and Thermotherapeutic treatments on Pepino mosaic virus in Tomato Seed. Plant Dis. 94:325-328.
(5) Ling, K.-S., Wintermantel, W.M., and Bledsoe, M. 2008. Genetic Composition of Pepino mosaic virus Population in North American Greenhouse Tomatoes. Plant Dis. 92:1683-1688.
(6) Mumford, R.A., and Jones, R.A.C. 2005. Pepino mosaic virus. Descriptions of plant viruses. No. 411. CMI/AAB.

-

-

-

96, 480, 960 reactions

Nunc-Immuno Plates MaxiSorp F96, certified;
pNPP blister-packed tablets (5 or 20 mg);
Washing buffer (PBST) (tablets or powder);
Substrate buffer (5x);
Conjugate buffer (10x);
Coating buffer (tablets);
Extraction buffer "General" (10x);
Negative control;
PePMV positive control;
PepMV Pepino mosaic virus Conjugate;
PePMV Pepino mosaic virus IgG;

-